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KMID : 0878220120240010054
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Journal of Sasang Constitutional Medicine
2012 Volume.24 No. 1 p.54 ~ p.65
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Effects of TAM (Taraxacum mongolicum) on Th2 Cytokine Production in MC/9 Mast Cells
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Jang Moon-Hee
Choi Jae-Song
Bae Na-Young
Ahn Teak-Won
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Abstract
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Objective: The purpose of this study is to investigate the effects of TAM (Taraxacum mongolicum) on Th2 cytokine production in MC/9 mast cells.
Methods: The effects of TAM was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR.
Results: 1) TAM inhibited the IL-4 production significantly in comparison to PI-control group at concentration of 50㎍/mL,100㎍/mL,200㎍/mL. 2) TAM inhibited the IL-13 production significantly in comparison to PI-control group at concentration of 50㎍/mL,100㎍/mL,200㎍/mL 3) TAM inhibited the IL-4 mRNA expression significantly in comparison to PI-control group at concentration of 100㎍/mL. 4) TAM inhibited the IL-5 mRNA expression significantly in comparison to PI-control group at concentration of 50㎍/mL,100㎍/mL 5) TAM inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100㎍/mL. 6) TAM inhibited the IL-13 mRNA expression significantly in comparison to PI-control group at concentration of 100㎍/mL.
Conclusions: These results indicate that TAM (Taraxacum mongolicum) has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.
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KEYWORD
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TAM (Taraxacum mongolicum), Cytokine, MC/9 Mast Cell, Atopic Dermatitis
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